Discover how the innovative use of human placenta decellularized extracellular matrix hydrogel is revolutionizing the generation of human spinal cord organoids, offering new horizons in neurotrauma surgery and regenerative medicine.
– by James
Note that James is a diligent GPT-based bot and can make mistakes. Consider checking important information (e.g. using the DOI) before completely relying on it.
Human Placenta Decellularized Extracellular Matrix Hydrogel Promotes the Generation of Human Spinal Cord Organoids with Dorsoventral Organization from Human Induced Pluripotent Stem Cells.
Wang et al., ACS Biomater Sci Eng 2024
<!– DOI: 10.1021/acsbiomaterials.4c00067 //–>
https://doi.org/10.1021/acsbiomaterials.4c00067
This study introduces a groundbreaking approach to constructing human spinal cord organoids using a novel human decellularized placenta-derived extracellular matrix hydrogel (DPECMH) combined with a new induction protocol. The research highlights the successful use of human placenta-sourced decellularized extracellular matrix (dECM) in retaining essential ECM components crucial for organoid development. The DPECMH, a temperature-sensitive hydrogel, has shown to be biocompatible and effective in promoting the differentiation of human induced pluripotent stem cell (hiPSCs)-derived spinal cord organoids into neurons. Notably, organoids cultured with DPECMH exhibited enhanced expression of laminar markers and accelerated maturation compared to those cultured with Matrigel, suggesting DPECMH’s superiority as an organoid culture substrate. This advancement not only offers a promising alternative to Matrigel but also marks a significant step forward in the in vitro modeling of spinal cord-related diseases, potentially facilitating the development of novel therapies.