This study investigates the interaction between multi-walled carbon nanotubes (MWCNTs) and a common allergen, house dust mites, in the context of potential inhalation exposure in industries like construction and aerospace. The research focuses on the formation of an allergen corona on the surface of MWCNTs when exposed to house dust mite extract and lung fluid proteins. Using techniques such as gel electrophoresis, western blotting, and proteomics, the study reveals that the corona is predominantly composed of der p 2, a protein known to trigger allergic responses to house dust mites. Notably, der p 2 remains attached to the MWCNTs even after exposure to lung fluid proteins, suggesting a high concentration of this allergen on the MWCNT surface. This finding is significant as it highlights the potential for MWCNTs to exacerbate allergic lung diseases by carrying allergens like der p 2. The research lays the groundwork for future cellular and in vivo studies aimed at understanding the mechanisms by which nanomaterials may worsen allergic conditions.