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Cortical brain organoid slices (cBOS) for the study of human neural cells in minimal networks.
Petersilie et al., iScience 2024
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https://doi.org/10.1016/j.isci.2024.109415
This study introduces cortical brain organoid slices (cBOS) cultured at the air-liquid interface from cortical organoids derived from human pluripotent stem cells. The cBOS exhibit mature neurons and astrocytes in a complex architecture, demonstrating their potential as a model for studying human neurodevelopment and disorders. Through whole-cell patch-clamp techniques, the researchers observed synaptic inputs and action potential firing in neurons. Importantly, they found that spontaneous intracellular calcium signals in cBOS could evolve into synchronous large-scale oscillations with specific pharmacological manipulations. Additionally, simulating transient energy restriction, akin to ischemic brain conditions, caused reversible intracellular calcium loading and a decline in neuronal ATP, which could be monitored using a specific sensor, ATeam1.03YEMK. This study highlights cBOS as a valuable tool for investigating the morphological and functional properties of human neural cells within minimal networks and exploring cellular damage mechanisms during brain ischemia.