Explore the groundbreaking identification of unique stromal-like cells in histiocytic lesions, offering a promising new in vitro model for drug testing and advancing our understanding of cellular phenotypes in medical genetics.
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Identification and characterization of stromal-like cells with CD207+/low CD1a+/low phenotype derived from histiocytic lesions – a perspective in vitro model for drug testing.
Śmieszek et al., BMC Cancer 2024
<!– DOI: 10.1186/s12885-023-11807-0 //–>
https://doi.org/10.1186/s12885-023-11807-0
New Information: The study presents newly characterized cellular models derived from histiocytic lesions in pediatric patients, which can be used for research on histiocytoses and drug testing. These models are named RAB-1, HAN-1, and CHR-1.
Importance: The development of these cellular models addresses the lack of pre-clinical in vitro models for histiocytoses, facilitating the study of molecular pathways and drug responses in these rare disorders.
Contribution to Literature: The study contributes to the current literature by providing a detailed characterization of the cytophysiological features of these cells, including their molecular phenotype, cytogenetic profile, morphology, ultrastructure, viability, mitochondrial activity, proliferation, and sensitivity to drugs.
The cells exhibit a heterogeneous molecular phenotype and morphology, expressing both dendritic cell markers (CD1a/CD207) and mesenchymal markers (VIM and OPN). They maintain viability and metabolic activity across cultures, with a well-developed mitochondrial network. Each cell line has a unique transcriptome profile, revealing potential new non-coding RNA biomarkers for histiocytoses. Additionally, the cells displayed varying sensitivity to the drugs vemurafenib and trametinib, which could inform therapeutic strategies.