Explore the cutting-edge of neurosurgical oncology as we delve into the methodological intricacies and analytical advancements shaping the future of intra-operative microdialysis.
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Methodological and analytical considerations for intra-operative microdialysis.
Riviere-Cazaux et al., Fluids Barriers CNS 2023
DOI: 10.1186/s12987-023-00497-2
Summary of Intra-operative High Molecular Weight Microdialysis in Glioma Surgery
What’s New: The study introduces the use of intra-operative high molecular weight (HMW) microdialysis with a 100 kDa catheter and a variable flow rate pump for sampling interstitial fluid during glioma surgeries. This method allows for the collection and analysis of metabolites and drugs directly from the central nervous system (CNS) in real-time during an operation.
Importance: This technique offers a novel approach to study the CNS microenvironment in gliomas, which could lead to better understanding of tumor metabolism and the effects of drugs during surgery.
Contributions to Literature: The research provides insights into methodological and analytical considerations for intra-operative HMW microdialysis. It demonstrates that the type of perfusate used can influence the metabolites detected, and that local metabolic changes can be induced by delivering substances like lactate through the perfusate. This suggests potential for pharmacodynamic studies of drugs during surgery.
Results: The trial was conducted during 15 glioma resections in 14 patients, with microdialysates analyzed for metabolomics using ultra-performance liquid chromatography tandem mass spectrometry. It was found that various drugs administered peri-operatively could be detected from a single 10-minute microdialysate sample.
Methodological Considerations: The study discusses the impact of albumin and lactate in perfusates on metabolite analysis and highlights the need to consider these variables during and after the procedure.
Registration: The trial is registered under NCT04047264.