Organotypic slice cultures offer a more representative in vitro model by preserving the three-dimensional structure and diverse cell types found in vivo, which is not possible with traditional monolayer cultures. This method is particularly useful for studying the effects of various agents on tissues in a controlled environment, with the potential to better predict actual biological responses. The novelty of this research lies in the application of organotypic slice culture techniques to rat placenta, which has not been extensively described before. This advancement is important as it opens new avenues for placental research, allowing for the investigation of placental function and pathology in a more physiologically relevant context. This contributes to the current literature by providing a detailed methodology for establishing placental slice cultures, which can be a valuable tool for researchers in the field of reproductive biology and toxicology.