Discover the natural remedy hidden in peonies as we delve into how Paeoniflorin, a compound from the Paeonia lactiflora plant, emerges as a promising anti-inflammatory agent in the battle against osteoarthritis.
– by James
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Paeoniflorin, the Main Monomer Component of Paeonia lactiflora, Exhibits Anti-inflammatory Properties in Osteoarthritis Synovial Inflammation.
Chen et al., Chin J Integr Med 2023
DOI: 10.1007/s11655-023-3653-9
Summary of Paeoniflorin’s Mechanism on Osteoarthritis Synovial Inflammation
What’s New: This study investigates the anti-inflammatory effects of paeoniflorin (PF) on osteoarthritis (OA) synovial inflammation, using a combination of network pharmacology and experimental pharmacology approaches.
Importance: The research identifies potential targets and pathways through which PF may exert its effects, providing insights into its therapeutic potential for OA.
Contribution to Literature: The study bridges the gap between computational predictions and experimental validation, offering a comprehensive analysis from potential targets to molecular mechanisms.
Key Findings:
- 26 co-targeted genes between OA and PF were identified.
- Gene Ontology (GO) analysis suggested these genes are involved in processes like cellular response to hypoxia and LPS-mediated signaling.
- Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated involvement in the HIF-1 and TNF signaling pathways.
- Protein-protein interaction (PPI) network highlighted TP53, TNF, and CASP3 as hub genes.
- Molecular docking supported PF’s interaction with TNF.
- Cell counting kit-8 (CCK-8) assay confirmed PF’s non-toxicity at concentrations up to 50 µmol/L on human OA fibroblast-like synoviocytes (FLS).
- PF significantly reduced the expression of pro-inflammatory markers (IL-1β, IL-6, TNF-α, MMP13, and ADAMTS5) in LPS-induced OA FLS.
Conclusion: PF demonstrates a significant anti-inflammatory effect in OA synovial inflammation, with no observed toxicity at relevant concentrations, supporting its potential as a therapeutic agent for OA.